Sequencing and comparison of Drosophila melanogaster vitelline membrane cDNA clones

  • 52 Pages
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  • English
Drosophila melanogaster., Molecular clo
Statementby Michael Kenneth White.
ContributionsBoston College. Dept. of Biology.
The Physical Object
Paginationv, 52 leaves :
ID Numbers
Open LibraryOL16576320M

Comparative analysis of the sequence and structure of two Drosophila melanogaster genes encoding vitelline membrane proteins. Scherer LJ(1), Harris DH, White MK, Steel LF, Jin J, Petri WH. Author information: (1)Department of Biology, Boston College, Chestnut Hill, MA Cited by: 3.

Two Drosophila melanogaster vitelline membrane protein-encoding genes (VM), located at polytene band positions 26A and 34C, have been cloned and comparatively characterized at the nucleotide ce analysis of genomic and cDNA clones for the two genes, VM26A.1 and VM34C.1, indicates that both are similarly organized with a central highly conserved domain [Scherer Cited by: 3.

Comparison of the deduced aa sequence for the D. melanogaster clone with that for the human B cDNA clone (Griffith et al, ) and the murine B cDNA clones (Ohosone et al., a,b; Van Dam et al., ) revealed significant identity at the aa level, with the N-terminal portion the most conserved (Fig.

3).Cited by: 6. sive clone-based sequence and a high-quality bacterial artificial chromo- The Drosophila genome is Mb in some physical map. Efforts are. Two Drosophila vitelline membrane (VM) genes located at polytene band positions 26A and 34C have been characterized at the nucleotide level.

Sequence comparison of the two genes VM26A.1 and VM34C.1 has revealed a similar base pair region centrally located in the coding regions of both by:   Drosophila Genomics Resource Center cDNA clones For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

Isolation and chromosomal location of putative vitelline membrane genes in Drosophila melanogaster. EMBO J.

4: – EMBO J. 4: – PubMed Google Scholar. A Drosophila expressed sequence tag, LD (BDGP) that extended the 5′ end of the cDNA by a further 80 bases was also identified.

The five overlapping cDNA clones encode a amino-acid open reading frame (Figure 2) within a kb transcript, in agreement with a transcript observed on Northern blots (data not shown). Several cytoplasmic factors have been identified that control the dynamics of actin filaments in microvilli.

However, it remains unclear whether the plasma membrane participates actively in microvillus formation. In this paper, we analyze the Sequencing and comparison of Drosophila melanogaster vitelline membrane cDNA clones book of Drosophila melanogaster cadherin Cad99C in the microvilli of ovarian follicle cells.

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data. Matthews BB, et al. G3 (Bethesda) Jun 24; The Release annotation of Drosophila melanogaster heterochromatin. Smith CD, et al. Science Jun 15; Sequence finishing and mapping of Drosophila melanogaster heterochromatin.

Hoskins RA, et al. Science Clones corresponding to two distinct A1 and A2 chorion genes have been isolated from a cDNA library in Drosophila melanogaster and characterized by hybrid-selected translation and blotting-hybridization analysis.

These sequences detectably cross hybridize, thus indicating that at least some chorion genes in the fruit fly are homologous. The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans.

We have determined the nucleotide sequence of nearly all of the ∼megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun. Source for merge of CkIIα anon-WO was sequence comparison (date).

(FlyBase, ) Drosophila Casein kinase II α and β subunits can rescue the lethality of siae deleted for both genes. About Drosophila melanogaster. Drosophila melanogaster is a cosmopolitan species of fruitfly that has been used as a model organism for over a hundred years, particularly with respect to genetics and developmental biology.

It was the second metazoan (the first being Caenorhabditis elegans) to have its genome sequenced [1], and was one of 12 fruitfly genomes included in a large comparative. A contiguous sequence of nearly 3 Mb from the genome of Drosophila melanogaster has been sequenced from a series of overlapping P1 and BAC clones.

This region covers 69 chromosome polytene bands on chromosome arm 2L, including the genetically well-characterized “ Adh region.” A computational analysis of the sequence predicts protein-coding genes, 11 tRNAs, and 17. Model Organism: Drosophila melanogaster Drosophila melanogaster: early embryogenesis The Drosophila egg is the shape of a sausage.

It has a micropyle at the anterior end (site of sperm entry). With fertilization, the fusion of nuclei is followed by rapid mitotic divisions (90 minutes) and no cleavage.

Description Sequencing and comparison of Drosophila melanogaster vitelline membrane cDNA clones PDF

A syncytium is formed (many nuclei/common. Drosophila Gene Collection. DGC Release ; After careful analysis of o ESTs to find full-length cDNA clones, including verification of the 3' ends of these clones, we have selected 5, non-redundant cDNA clones for single colony purification and re-arraying (see What is the Drosophila Gene Collection.

for more information). This is not a complete set of genes for the Drosophila. FlyBase: a database for drosophila genetics and molecular biology. genome as a prelude to sequencing the human genome, and (ii) to provide a com-plete, high-quality genomic sequence to the Drosophila research community so as to advance research in this important model organism.

WGS sequencing is an effective and effi-cient way to sequence the genomes of pro-karyotes, which are generally between and 6 Mb in.

Details Sequencing and comparison of Drosophila melanogaster vitelline membrane cDNA clones FB2

Annotation overview. The Drosophila Heterochromatin Genome Project has generated 16 megabases (Mb) of finished or near-finished heterochromatin sequence from D. melanogaster, as well as 8 Mb of draft whole genome shotgun (WGS) heterochromatic assemblies (8, 9).We performed computational and manual curation to produce the Release annotation of this 24 Mb of heterochromatin sequence.

Drosophila Genomics Resource Center cDNA clones For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA clones for two Drosophila vitelline membrane genes have been identified on the basis of: (i) stage and tissue specificity of transcription and (ii) size and amino acid content of the. The Drosophila dec-1 gene produces three proproteins required for female fertility and eggshell assembly.

Download Sequencing and comparison of Drosophila melanogaster vitelline membrane cDNA clones FB2

The three proproteins are distinguished by their C termini. Fc, the most abundant proprotein, is cleaved within the vitelline membrane to three mature derivatives in a developmentally regulated manner.

To define sequences within fc that are critical for its function, we created wild. The BDGP/HHMI full-length cDNA EST project's goal is to sequence the 5′ ends of 80 cDNA clones made from high quality Drosophila cDNA libraries.

The long-term goal is to generate the full-length sequence of representative clones for each gene and compare them to the genomic sequence to generate a transcript map of the genome.

This drosophila cDNA will be compared against the human genome to attempt to find a human homolog to the genes of drosophila, thus, showing the importance of drosophila as a model system for human disease.

Methods and Materials Taken from: “cDNA Isolation and Analysis: Identifying potential Drosophila melanogaster models of human disease.”.

We present the sequence of a contiguous Mb of DNA extending from the tip of the X chromosome of Drosophila this sequence, we predict protein coding genes, of which 94 had been sequenced already in the course of studying the biology of their gene products, and examples of 12 different transposable elements.

The sequence of the cDNA clone has been deposited into GenBank with accession number AF The open reading frame of the cDNA clone codes for a protein of amino acids with a potential signal sequence at the N-terminus (amino acids 1–16 MMLFVVASALVALA QT, Perlman and Halvorson, ) (Fig.

Vitelline envelope genes from the mosquito Aedes aegypti were analyzed with respect to their DNA sequences, genomic representation, temporal and spatial expression profiles and response to hydroxyecdysone.

Genomic clones of three vitelline envelope genes, 15a-1, 15a-2 and 15a-3 were isolated. Southern analysis indicates that all three genes are represented by a single copy in the. By using the cDNA clone containing the sequence for the L1 ribosomal protein gene of Xenopus laevis as probe (1), we have isolated positive phages from a Drosophila melanogaster genonmic library.

The cDNA sequence of the D. melanogaster sialic acid phosphate synthase (DmSAS) (Figure 1) is predicted to encode a protein of amino acids, with a molecular weight of 41 kDa.

Homology alignments between the Drosophila, human, and bacterial sialic acid synthase enzymes (Figure 2) show that the proteins are homologous over their entire length. FCS gene was similar to Drosophila vitelline membrane protein genes, with % similarity in nucleotide sequence and % similarity in amino acid sequence.

The conserved hydrophobic regions from several vitelline membrane proteins were compared. Another cDNA clone, 1D, was isolated from the cDNA library screened with the FCS gene.Among the members of the Drosophila melanogaster vitelline membrane protein gene family, VM32E has the unique feature of being a component of both the vitelline and the endochorion layers.

The publication of the complete euchromatic portion of the Drosophila melanogaster (Drm) genome by Adams et al.() enabled the analysis of the full repertoire of Drm G protein-coupled receptors (GPCRs) for the first timeThe Drm genome encodes about GPCR genes, including at least 21 GPCRs for classic neurotransmitters and neuromodulators and between 39 and 45 peptide .